Journal: Genes to Cells

Article Title: Molecular Mechanism of Caspase‐8–Dependent Interleukin‐18 Activation in Pancreatic Cancer Cells Induced by 5‐Fluorouracil and Nutrient Starvation

doi: 10.1111/gtc.70111

Figure Lengend Snippet: Induction of cleaved IL‐18 by 5‐FU treatment in human pancreatic cancer cell lines. (A) MIA PaCa‐2 and Panc‐1 cells were cultured in low‐nutrient culture medium and treated with 5‐FU at the indicated concentrations for 48 h. Whole‐cell lysates were analyzed by western blotting with anti‐IL‐18 mAbs. β‐Actin was used as a loading control. (B) Culture supernatants from MIA PaCa‐2 cells treated with 5‐FU for 48 h were analyzed by IL‐18 sandwich ELISA; absorbance (450–620 nm) is shown. * p < 0.05.

Article Snippet: Human pancreatic cancer cell lines (MIA PaCa‐2 and Panc‐1), human colorectal cancer cell line (HCT116), and human cervical cancer cell line (HeLa) were purchased from the American Type Culture Collection.

Techniques: Cell Culture, Western Blot, Control, Sandwich ELISA

Journal: Genes to Cells

Article Title: Molecular Mechanism of Caspase‐8–Dependent Interleukin‐18 Activation in Pancreatic Cancer Cells Induced by 5‐Fluorouracil and Nutrient Starvation

doi: 10.1111/gtc.70111

Figure Lengend Snippet: Cleaved IL‐18 induction by 5‐FU in cancer cells other than pancreatic cancer cells. (A) Representative images of HCT116 and HeLa cells treated with 5‐FU for 48 h in low‐nutrient culture medium (×10). 5‐FU was used at 10 μg/mL for HCT116 cells and 50 μg/mL for HeLa cells. Detached HCT116 cells were observed only after 5‐FU treatment. (B) HCT116 cells treated with 5‐FU were collected as attached or detached fractions; all other samples were collected as whole cells. Lysates were analyzed by western blotting with the indicated antibodies. β‐Actin was used as a loading control.

Article Snippet: Human pancreatic cancer cell lines (MIA PaCa‐2 and Panc‐1), human colorectal cancer cell line (HCT116), and human cervical cancer cell line (HeLa) were purchased from the American Type Culture Collection.

Techniques: Western Blot, Control

Journal: Genes to Cells

Article Title: Molecular Mechanism of Caspase‐8–Dependent Interleukin‐18 Activation in Pancreatic Cancer Cells Induced by 5‐Fluorouracil and Nutrient Starvation

doi: 10.1111/gtc.70111

Figure Lengend Snippet: Proposed model of pyroptosis induction in pancreatic cancer cells. Nutrient starvation induces caspase‐8–dependent cell pyroptosis accompanied by IL‐18 and GSDMD cleavage. 5‐FU increases the number of cells undergoing pyroptosis. Increased release of active IL‐18 can ultimately lead to chronic inflammation in the tumor environment.

Article Snippet: Human pancreatic cancer cell lines (MIA PaCa‐2 and Panc‐1), human colorectal cancer cell line (HCT116), and human cervical cancer cell line (HeLa) were purchased from the American Type Culture Collection.

Techniques: